| 產(chǎn)品編號 | bs-0541R |
| 英文名稱 | CAMK2A + CAMK2B + CAMK2D Rabbit pAb |
| 中文名稱 | 鈣/鈣調(diào)素依賴蛋白激酶2b/2γ抗體 |
| 別 名 | CaMK2 beta+gamma+delta; CaMK2 beta; CaMK2 gamma; CaMK2 delta; CAMK2G; Calcium/calmodulin dependent protein kinase(CaM kinase) II beta; Calcium/calmodulin dependent protein kinase II beta; Calcium/calmodulin dependent protein kinase IIB; Calcium/calmodulin |
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Specific References (2) | bs-0541R has been referenced in 2 publications.
[IF=3.457] Li W et al. Upregulation?of?MMP-9?and?CaMKII?prompts?cardiac?electrophysiological?changes?that?predispose?denervated?transplanted?hearts?to?arrhythmogenesis?after?prolonged cold ischemic storage. Biomed Pharmacother.?2019 Feb 20;112:108641. WB ; Rat.
[IF=2.088] Wen Jiang. et al. Hirsutine ameliorates myocardial ischemia-reperfusion injury through improving mitochondrial function via CaMKII pathway. CLIN EXP HYPERTENS. 2023;45(1):Article: 2192444 WB ; Rat.
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| 研究領(lǐng)域 | 腫瘤 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 細(xì)胞周期蛋白 激酶和磷酸酶 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 53-73 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CaMK2 beta/ gamma/ delta: 1-100/479 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Eight transcript variants encoding eight distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]. Function: Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplamic reticulum Ca(2+) transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplamic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2. Subunit: CAMK2 is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other. Interacts with SYNGAP1 and CAMK2N2 (By similarity). Interacts with MPDZ. Subcellular Location: Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Note=In slow-twitch muscle, evenly distributed between longitudinal SR and junctional SR. Tissue Specificity: Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Expressed in skeletal muscle. Post-translational modifications: Autophosphorylation of Thr-287 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-287 locks the kinase into an activated state. Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain. SWISS: Q9UQM7 Gene ID: 818 Database links: Entrez Gene: 815 Human Entrez Gene: 816 Human Entrez Gene: 817 Human Entrez Gene: 108058 Mouse Entrez Gene: 12322 Mouse Entrez Gene: 12323 Mouse Omim: 114078 Human Omim: 607707 Human Omim: 607708 Human SwissProt: Q13554 Human SwissProt: Q13557 Human SwissProt: Q9UQM7 Human SwissProt: P11798 Mouse SwissProt: P28652 Mouse SwissProt: Q6PHZ2 Mouse CaMK2b也是絲/蘇氨基酸激酶的一種，同屬于Ca++/鈣調(diào)素依賴性蛋白質(zhì)激酶家族成員。鈣／鈣調(diào)素依賴性蛋白激酶Ⅱb（CaMKⅡb）廣泛分布于中樞神經(jīng)系統(tǒng)內(nèi)，參與調(diào)節(jié)神經(jīng)遞質(zhì)的合成釋放、信號轉(zhuǎn)導(dǎo)和突觸可塑性的形成以及磷酸化蛋白 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CaMK2b) Polyclonal Antibody, Unconjugated (bs-0541R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CAMK2A + CAMK2B + CAMK2D) Polyclonal Antibody, Unconjugated (bs-0541R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CAMK2A + CAMK2B + CAMK2D) Polyclonal Antibody, Unconjugated (bs-0541R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CaMK2b) Polyclonal Antibody, Unconjugated (bs-0541R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
4% Paraformaldehyde-fixed SH-SY5Y(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (CAMK2A + CAMK2B + CAMK2D) polyclonal Antibody, unconjugated (bs-0541R) 1:50, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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