| 產(chǎn)品編號 | bs-0582R |
| 英文名稱 | Cyclooxygenase 1 Rabbit pAb |
| 中文名稱 | 前列腺素氧化環(huán)化酶1抗體 |
| 別 名 | Cyclooxygenase-1; COX-1; COX 1; COX1; Cyclooxygenase1; Prostaglandin-endoperoxide synthase-1; prostaglandin-endoperoxide synthase 1; PTGS-1; Prostaglandin G/H synthase 1 precursor; prostaglandin G/H synthase 1 isoform 1 precursor; Prostaglandin H2 synthas |
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Specific References (4) | bs-0582R has been referenced in 4 publications.
[IF=2.86] Li, Wei, et al. "Combined effects of cyclooxygenase-1 and cyclooxygenase-2 selective inhibitors on ovarian carcinoma in vivo." International journal of molecular sciences 12.1 (2011): 668-681 WB ; Mouse.
[IF=2.38] Hu, Ruiqi, et al. "The expression of prostaglandin-E2 and its receptor in the oviduct of Chinese brown frog (Rana dybowskii)." Prostaglandins & Other Lipid Mediators (2016). IHC-P ; Others.
[IF=2.253] Chen Z et al. Seasonal expressions of prostaglandin E synthases and receptors in the prostate of the wild ground squirrel (Spermophilus dauricus). Prostaglandins and Other Lipid Mediators 148 (2020) 106412. IHC-P ; squirrel.
[IF=2.253] Wang Y et al. Seasonal expressions of COX-1, COX-2 and EP4 in the uteri of the wild Daurian ground squirrels (Spermophilus dauricus). Prostaglandins Other Lipid Mediat. 2019 Aug;143:106343. IHC&WB ; squirrel.
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| 研究領域 | 腫瘤 心血管 免疫學 信號轉(zhuǎn)導 合成與降解 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Pig,Sheep,Cow,Dog) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 63 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Cyclooxygenase 1: 151-250/599 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This is one of two genes encoding similar enzymes that catalyze the conversion of arachinodate to prostaglandin. The encoded protein regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. The protein may promote cell proliferation during tumor progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2012]. Function: May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells. Subunit: Homodimer. Subcellular Location: Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Similarity: Belongs to the prostaglandin G/H synthase family. Contains 1 EGF-like domain. SWISS: P23219 Gene ID: 5742 Database links: Entrez Gene: 5742 Human Entrez Gene: 19224 Mouse Omim: 176805 Human SwissProt: P23219 Human SwissProt: P22437 Mouse Unigene: 201978 Human Unigene: 275434 Mouse Unigene: 44404 Rat 合成與降解（Synthesis and Degradation） COX1 前列腺素氧化環(huán)化酶-是前列腺素合成的關(guān)鍵酶之一，為誘導性酶，他參與細胞對生長因子、腫瘤促進因子和細胞因子的反應，這些因子均能誘導COX1表達，它也參與前列腺素的合成。 |
| 產(chǎn)品圖片 |
Sample:
NIH/3T3(Mouse) Cell Lysate at 30 ug
A431(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Huvec(Human) Cell Lysate at 30 ug
Primary: Anti-Cyclooxygenase 1 (bs-0582R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kD
Observed band size: 63 kD
Tissue/cell: human laryngo carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-COX-1 Polyclonal Antibody, Unconjugated(bs-0582R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
