| 產(chǎn)品編號(hào) | bs-1278R |
| 英文名稱(chēng) | 8-OHdG (DNA/RNA Damage) Rabbit pAb |
| 中文名稱(chēng) | 8-羥基脫氧鳥(niǎo)苷抗體 |
| 別 名 | 8-hydroxy-2'-deoxyguanosine; 2'-Deoxy-8-oxoguanosine; 7, 8-Dihydro-8-oxo-2'-deoxyguanosine; 8-Oxo-7, 8-dihydro-2'-deoxyguanosine; 8-Oxo-7, 8-dihydrodeoxyguanosine; 2'-Deoxy-8-oxo-D-guanosine; 8-Oxo-7, 8-dihydro-2μ-deoxyguanosine, 8-Oxo-dG; 2'-Deoxy-8-hydr 8-OH-脫氧鳥(niǎo)嘌呤; 8-羥基-2'-脫氧鳥(niǎo)嘌呤; 2'-脫氧-7,8-二氫-8-氧代鳥(niǎo)苷; 8-羥基-2-脫氧鳥(niǎo)苷; 8-羥基脫氧鳥(niǎo)苷; 2'-脫氧-8-氧代鳥(niǎo)嘌呤; 8-氧-2'-脫氧鳥(niǎo)苷; 核酸氧化8-羥基脫氧鳥(niǎo)苷; 8-羥基-2′-脫氧鳥(niǎo)苷; |
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Specific References (79) | bs-1278R has been referenced in 79 publications.
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| 產(chǎn)品類(lèi)型 | 小分子抗體 |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 染色質(zhì)和核信號(hào) 表觀(guān)遺傳學(xué) Alzheimer's |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Rat,Species independent |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 0.283 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated 8-OHdG |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
8-Hydroxydeoxyguanosine (8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer's disease. Function: Oxoguanine 8 (8-Oxoguanine) is a mutagenic oxidative damage product of guanine. Guanine is the main target for reactive oxygen species in DNA, with 8-oxoguanine being the most frequent base lesion. Thus formation of 8-oxoguanine is an important biomarker of oxidative damage to DNA. It is primarily repaired by the DNA glycosylase OGG1. 8-OHDG作為內(nèi)源性及外源性因素對(duì)DNA氧化損傷作用的生物標(biāo)志物，是一個(gè)極有前途的指標(biāo)，通過(guò)8-OHDG的檢測(cè)可以評(píng)估體內(nèi)氧化損傷和修復(fù)的程度，氧化應(yīng)激與DNA損傷的相互關(guān)系，對(duì)研究退行性疾病、衰老機(jī)制、癌發(fā)生機(jī)制、環(huán)境毒物與氧化應(yīng)激的關(guān)系等均有重要的意義，也可以用來(lái)評(píng)價(jià)抗氧化劑治療DNA氧化損傷的效果。已經(jīng)證實(shí)，飲食、超氧化物歧化酶、Melatonin等對(duì)8-OHDG水平具有調(diào)節(jié)作用。近期日本學(xué)者制成一種抗氧化作用雞尾酒，由葵花籽、綠茶和維生素c組成，證實(shí)了對(duì)FeC12誘導(dǎo)的8-OHDG異常增加有回調(diào)作用。隨著研究的深入，8-OHDG的應(yīng)用范圍必將越來(lái)越廣，但到目前為止，各種檢測(cè)方法都還需進(jìn)一步完善，因而深入開(kāi)展這項(xiàng)研究工作將有十分重要的實(shí)際意義。 |
| 產(chǎn)品圖片 |
Tissue/cell: rat brain tissue(left panel was injury,Right panel was normal); 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-8-OHdG Polyclonal Antibody, Unconjugated(bs-1278R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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