| 產(chǎn)品編號(hào) | bs-5074R |
| 英文名稱 | Insig1 Rabbit pAb |
| 中文名稱 | 胰島素誘導(dǎo)基因1抗體 |
| 別 名 | CL 6; CL6; INSIG 1; INSIG 1 membrane protein; INSIG1 membrane protein; Insulin induced gene 1; Insulin induced gene 1 protein; insulin induced gene-1; insulin induced gene1; MGC1405; INSI1_HUMAN. |
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Specific References (4) | bs-5074R has been referenced in 4 publications.
[IF=5.168] Yu et al. LncRNA as ceRNAs may be involved in lactation process. (2017) Oncotarget. 8:98014-98028 IF(IHC-P) ; Goat.
[IF=4.192] Huang L et al. CRISPR/Cas9 Based Knockout of miR-145 Affects Intracellular Fatty Acid Metabolism by Targeting INSIG1 in Goat Mammary Epithelial Cells. J Agric Food Chem
. 2020 May 6;68(18):5138-5146. WB ; goat.
[IF=3.9] Wang et al. MicroRNA-26a/b and their host genes synergistically regulate triacylglycerol synthesis by targeting the INSIG1 gene. (2016) RNA.Bio. 13:500-10 WB ; goat.
[IF=1.58] Kim et al. Relaxin Receptor RXFP1 and RXFP2 Expression in Ligament, Tendon, and Shoulder Joint Capsule of Rats. (2016) J.Korean.Med.Sci. 31:983-8 IHC,WB ; Rat.
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| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 糖尿病 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Rabbit,Pig,Sheep,Cow,Chicken,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 30 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Insig1: 101-200/277 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Oxysterols regulate cholesterol homeostasis through liver X receptor (LXR) and sterol regulatory element binding protein (SREBP) mediated signaling pathway. Insig1 is an insulin-induced gene. It encodes an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. Insig1 binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. It may also play a role in growth and differentiation of tissues involved in metabolic control, and may play a regulatory role during G0/G1 transition of cell growth. Function: Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligase, AMFR/gp78. May play a role in growth and differentiation of tissues involved in metabolic control. May play a regulatory role during G0/G1 transition of cell growth. Subunit: Binds to the SCAP-SREBF2 complex only in the presence of sterols. Interacts with RNF139. Interacts with HMGCR (via its SSD); the interaction, accelerated by sterols, leads to the recruitment of HMGCR to AMFR/gp78 for its ubiquitination by the sterol-mediated ERAD pathway. Interacts with AMFR/gp78 (via its membrane domain); the interaction recruits HMCR at the ER membrane for its ubiquitination and degradation by the sterol-mediated ERAD pathway. Subcellular Location: Endoplasmic reticulum membrane; Multi-pass membrane protein. Tissue Specificity: Expressed in all tissues tested with highest expression in the liver. Post-translational modifications: Ubiquitinated. Subsequent to sterol deprivation, the SCAP-SREBF2 complex becomes dissociated from INSIG1, is then ubiquitinated and degraded in proteasomes. Although ubiquitination is required for rapid INSIG1 degradation, it is not required for release of the SCAP-SREBP complex. Ubiquitinated by RNF139. Similarity: Belongs to the INSIG family. SWISS: O15503 Gene ID: 3638 Database links: Entrez Gene: 3638 Human Entrez Gene: 231070 Mouse Omim: 602055 Human SwissProt: O15503 Human SwissProt: Q8BGI3 Mouse Unigene: 520819 Human Unigene: 30221 Mouse Unigene: 772 Rat 胰島素誘導(dǎo)基因1在參與代謝控制的組織生長(zhǎng)和分化中發(fā)揮作用，在細(xì)胞生長(zhǎng)的G0/G1期的轉(zhuǎn)變過(guò)程中發(fā)揮調(diào)節(jié)作用. |
| 產(chǎn)品圖片 |
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-Insig1 (bs-5074R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 30 kD
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