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LAMP2 Rabbit pAb (bs-2379R)  
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50ul/1180.00元
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200ul/2800.00元
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產(chǎn)品編號(hào) bs-2379R
英文名稱(chēng) LAMP2 Rabbit pAb
中文名稱(chēng) 溶酶體相關(guān)膜蛋白2(CD107B)抗體
別    名 CD107 antigen-like family member B; CD107b; CD107b antigen; Igp110; Igp2; LAMP 2; LAMP 2C; LAMP B; LAMP-2; LAMP2_RAT; LAMPB; LGP110; Lysosomal associated membrane protein 2C; Lysosome associated membrane glycoprotein 2 precursor; Lysosome associated membr  
Specific References  (10)     |     bs-2379R has been referenced in 10 publications.
[IF=14.224] Ling Liu. et al. Polystyrene micro(nano)plastics damage the organelles of RBL-2H3 cells and promote MOAP-1 to induce apoptosis. J HAZARD MATER. 2022 Sep;438:129550  WB ;  Rat.  
[IF=6.048] Hao Liu. et al. Quercetin protects against palmitate-induced pancreatic β-cell apoptosis by restoring lysosomal function and autophagic flux. J NUTR BIOCHEM. 2022 May;:109060  WB,IF ;  Rat.  
[IF=6.025] Limei Yang. et al. The disruption of human trophoblast functions by autophagy activation through PI3K/AKT/mTOR pathway induced by exposure to titanium carbide (Ti3C2) MXene. FOOD CHEM TOXICOL. 2022 May;:113128  WB ;  Human.  
[IF=5.98] Luks, Lisanne, et al. "Novel insights into renal d-amino acid oxidase accumulation: propiverine changes DAAO localization and peroxisomal size in vivo." Archives of Toxicology (2016): 1-11.  IHC-P ;  Rat.  
[IF=5.23] Kameyama, Hirokazu, et al. "Iowa Mutant Apolipoprotein AI (ApoA-IIowa) Fibrils Target Lysosomes." Scientific Reports 6 (2016): 30391.  WB ;  Human.  
[IF=4.556] Woojin Na. et al. Aesculetin Inhibits Osteoclastic Bone Resorption through Blocking Ruffled Border Formation and Lysosomal Trafficking. Int J Mol Sci. 2020 Jan;21(22):8581  WB ;  Mouse.  
[IF=3.66] Iwao, Yasuhito, et al. "Liver atrophy after percutaneous transhepatic portal embolization occurs in two histological phases: Hepatocellular atrophy followed by apoptosis." World Journal of Hepatology 9.32 (2017): 1227.  IHC-P ;  Pig.  
[IF=3.274] Dong W et al. Polybrominated Diphenyl Ethers Quinone Induces NCOA4-mediated Ferritinophagy through Selectively Autophagic Degradation of Ferritin. Chem Res Toxicol. 2019 Nov 11.  WB ;  Rattus norvegicus.  
[IF=3.23] Sun, Qianqian, et al. "Factors that Affect Pancreatic Islet Cell Autophagy in Adult Rats: Evaluation of a Calorie-Restricted Diet and a High-Fat Diet."PLOS ONE 11.3 (2016): e0151104.  IHC-P ;  Rat.  
[IF=0] Iwao et al. Liver atrophy after percutaneous transhepatic portal embolization occurs in two histological phases: Hepatocellular atrophy followed by apoptosis. (2017) World.J.Hepatol. 9:1227-1238  IHC-P ;  Porcine.  
研究領(lǐng)域 腫瘤  心血管  細(xì)胞生物  信號(hào)轉(zhuǎn)導(dǎo)  干細(xì)胞  新陳代謝  線粒體  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
交叉反應(yīng) Human,Mouse,Rat
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1:20-50,ICC/IF=1:50-200,IP=1:20-50
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 45 kDa
檢測(cè)分子量 120
細(xì)胞定位 細(xì)胞漿 細(xì)胞膜 線粒體
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human LAMP2: 301-411/411 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The protein encoded by the LAMP2 gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands and is thought to play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of the gene produces two known products, LAMP2a and LAMP2b. Isoform LAMP2a is highly expressed in placenta, lung and liver and has low expression in brain and skeletal muscle. The isoform detected by this antibody, LAMP2b, is highly expressed in skeletal muscle less so in brain, placental, lung, kidney and pancreas and has very low expression in liver.

Function:
Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter-and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note=This protein shuttles between lysosomes, endosomes, and the plasma membrane.

Post-translational modifications:
O- and N-glycosylated; some of the N-glycans attached to Lamp-2 are polylactosaminoglycans.

Similarity:
Belongs to the LAMP family.

SWISS:
P13473

Gene ID:
3920

Database links:

Entrez Gene: 3920 Human

Entrez Gene: 16784 Mouse

Omim: 309060 Human

SwissProt: P13473 Human

SwissProt: P17047 Mouse

Unigene: 496684 Human

Unigene: 486 Mouse




產(chǎn)品圖片
25 ug total protein per lane of various lysates (see on figure) probed with LAMP2 polyclonal antibody, unconjugated (bs-2379R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Thyroid Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP2? Polyclonal Antibody, Unconjugated (bs-2379R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP2? Polyclonal Antibody, Unconjugated (bs-2379R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP2? Polyclonal Antibody, Unconjugated (bs-2379R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP2? Polyclonal Antibody, Unconjugated (bs-2379R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP2? Polyclonal Antibody, Unconjugated (bs-2379R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP2? Polyclonal Antibody, Unconjugated (bs-2379R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP2? Polyclonal Antibody, Unconjugated (bs-2379R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP2? Polyclonal Antibody, Unconjugated (bs-2379R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP2? Polyclonal Antibody, Unconjugated (bs-2379R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP2? Polyclonal Antibody, Unconjugated (bs-2379R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP2? Polyclonal Antibody, Unconjugated (bs-2379R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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