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PU.1/Spi1 Rabbit pAb (bs-19594R)  
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產(chǎn)品編號 bs-19594R
英文名稱 PU.1/Spi1 Rabbit pAb
中文名稱 造血轉(zhuǎn)錄因子PU.1抗體
別    名 31 kDa Transforming Protein; 31 kDa-transforming protein; Hematopoietic transcription factor PU.1; OF; SFPI1; SPI 1 proto oncogene; SPI A; SPI1_HUMAN; Spleen focus forming virus(SFFV) proviral integration oncogene spi1; Transcription factor PU.1.  
研究領(lǐng)域 免疫學(xué)  轉(zhuǎn)錄調(diào)節(jié)因子  b-淋巴細(xì)胞  表觀遺傳學(xué)  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應(yīng) Human (predicted: Mouse,Rat,Pig,Sheep,Cow,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 31 kDa
檢測分子量
細(xì)胞定位 細(xì)胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human PU.1/Spi1: 151-250/270 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The enzyme encoded by this gene catalyzes the elimination of inorganic triphosphate from dihydroneopterin triphosphate, which is the second and irreversible step in the biosynthesis of tetrahydrobiopterin from GTP. Tetrahydrobiopterin, also known as BH(4), is an essential cofactor and regulator of various enzyme activities, including enzymes involved in serotonin biosynthesis and NO synthase activity. Mutations in this gene result in hyperphenylalaninemia. [provided by RefSeq, Oct 2008]

Function:
Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.

Subunit:
Binds DNA as a monomer. Interacts with CEBPD and NONO (By similarity). Interacts with RUNX1 and SPIB. Interacts with GFI1; the interaction represses SPI1 transcriptional activity.

Subcellular Location:
Nucleus.

Similarity:
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.

SWISS:
P17947

Gene ID:
6688

Database links:

Entrez Gene: 6688 Human

Entrez Gene: 20375 Mouse

Entrez Gene: 366126 Rat

Omim: 165170 Human

SwissProt: P17947 Human

SwissProt: P17433 Mouse

SwissProt: Q6BDS1 Rat

Unigene: 502511 Human

Unigene: 1302 Mouse

Unigene: 212982 Rat



產(chǎn)品圖片
Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-PU.1 (bs-19594R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 31 kD
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