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NrCAM Rabbit pAb (bs-20119R)  
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產品編號 bs-20119R
英文名稱 NrCAM Rabbit pAb
中文名稱 神經細胞粘附分子NrCAM抗體
別    名 Neuronal cell adhesion molecule; Neuronal surface protein Bravo; Ng CAM related; Ng-CAM-related; NgCAM related cell adhesion molecule; NgCAM-related cell adhesion molecule; Nr CAM; Nr-CAM; Nrcam; NRCAM_HUMAN; NRCAM_MOUSE.  
研究領域 腫瘤  細胞生物  神經生物學  細胞類型標志物  細胞膜蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Rat (predicted: Mouse)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 141 kDa
檢測分子量
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from mouse NrCAM: 201-300/1256 <Extracellular>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Neuronal cell adhesion molecule (NrCAM) is a cell surface protein of the immunoglobulin (Ig) superfamily. NrCAM (also known as Bravo) contains six Ig domains, five fibronectin repeats, a transmembrane region and an intracellular domain. NrCAM is expressed in brain, spinal cord, peripheral nervous system and pancreas. In the spinal cord, NrCAM acts as a ligand for axonin-1 to guide commissural axons across the floor plate. NrCAM also acts as a ligand for F3 to control actin-dependent growth cone motility. NrCAM interacts with neurofascin and may facilitate the clustering of the cystoskeletal protein ankyrin G and the voltage-dependent sodium channel proteins at the node of Ranvier. NrCAM expression may play a role in the severity of certain types of tumors. NrCAM is overexpressed in high-grade astrocytomas, gliomas and glioblastoma tumor tissues. In the pancreas, NrCAM expression is upregulated in intraductal hyperplasia. Antisense NrCAM reduces the tumorigenic properties of human glioblastoma cells in vitro and slowed tumor growth in vivo. The gene encoding human NrCAM maps to chromosome 7q31.1-q31.2.

Function:
Cell adhesion, ankyrin-binding protein involved in neuron-neuron adhesion. May play a role in the molecular assembly of the nodes of Ranvier.

Subunit:
Probable constituent of a neurofascin/NRCAM/ankyrin-G complex. Interacts with GLDN/gliomedin. Interacts with MYOC.

Subcellular Location:
Cell membrane.

Tissue Specificity:
Detected in all the examined tissues. In the brain it was detected in the amygdala, caudate nucleus, corpus callosum, hippocampus, hypothalamus, substantia nigra, subthalamic nucleus and thalamus.

Similarity:
Belongs to the immunoglobulin superfamily.
L1/neurofascin/NgCAM family.
Contains 5 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

SWISS:
Q810U4

Gene ID:
319504

Database links:

Entrez Gene: 4897 Human

Entrez Gene: 534500 Cow

Entrez Gene: 319504 Mouse

Entrez Gene: 497815 Rat

Omim: 601581 Human

SwissProt: Q92823 Human

SwissProt: Q810U4 Mouse

SwissProt: P97686 Rat

Unigene: 21422 Human

Unigene: 208439 Mouse

Unigene: 10691 Rat



產品圖片
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Neuronal cell adhesion molecule; NrCAM) Polyclonal Antibody, Unconjugated (bs-20119R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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