| 產(chǎn)品編號 | bs-39011R |
| 英文名稱 | phospho-BHMT1(Thr45) Rabbit pAb |
| 中文名稱 | 磷酸化BHMT抗體 |
| 別 名 | BHMT1(phospho T45); BHMT1(phospho-Thr45); p-BHMT1(Thr45); betaine homocysteine methyltransferase; betaine homocysteine S methyltransferase 1; betaine homocysteine S methyltransferase; betaine--homocysteine S-methyltransferase 1; BHMT1; BHMT1_HUMAN; EC 2.1 |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 信號轉(zhuǎn)導(dǎo) 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 45 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human BHMT1 around the phosphorylation site of Thr45 : PW(p-T)PE |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008] Function: Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline. Subcellular Location: Cytoplasm. Tissue Specificity: Found exclusively in liver and kidney. Similarity: Contains 1 Hcy-binding domain. SWISS: Q93088 Gene ID: 635 Database links:
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| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded Human Kidney Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-BHMT1(Thr45) Polyclonal Antibody, Unconjugated (bs-39011R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRPand DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-BHMT1(Thr45) Polyclonal Antibody, Unconjugated (bs-39011R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRPand DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-BHMT1(Thr45) Polyclonal Antibody, Unconjugated (bs-39011R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRPand DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-BHMT1(Thr45) Polyclonal Antibody, Unconjugated (bs-39011R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRPand DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-BHMT1(Thr45) Polyclonal Antibody, Unconjugated (bs-39011R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRPand DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
