| 產(chǎn)品編號 | bsm-54451R |
| 英文名稱 | Fbx32 Recombinant Rabbit mAb |
| 中文名稱 | 泛素蛋白連接酶重組兔單抗 |
| 別 名 | Fbx32; 4833442G10Rik; AI430017; Atrogin 1; Atrogin-1; ATROGIN1; Atrophy gene 1; F box only protein 32; F-box only protein 32; F-box protein 32; FBX32_HUMAN; fbxo25; FBXO32; FLJ32424; MAFbx; MGC108443; MGC137646; MGC33610; Muscle atrophy F box; Muscle atro |
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Specific References (6) | bsm-54451R has been referenced in 6 publications.
[IF=8.713] Zhao-Bo Luo. et al. Fecal transplant from myostatin deletion pigs positively impacts the gut-muscle axis. ELIFE. 2023; 12: e81858 WB ; Mouse.
[IF=6.228] Xuzhu Lin. et al. Undercarboxylated osteocalcin and ibandronate combination ameliorates hindlimb immobilization-induced muscle wasting. J PHYSIOL-LONDON. 2023 Apr;: WB ; Human.
[IF=5.085] Shuang-Yan Chang. et al. miR-320 regulates myogenesis by targeting growth factor receptor-bound protein-2 and ameliorates myotubes atrophy. INT J BIOCHEM CELL B. 2022 Jun;147:106212 WB ; Mouse.
[IF=4.755] Zhang Lijiao. et al. Dysregulated myokines and signaling pathways in skeletal muscle dysfunction in a cigarette smoke–induced model of chronic obstructive pulmonary disease. FRONT PHYSIOL. 2022 Aug;0:1600 WB ; Mouse.
[IF=4.171] Xunyu Wei. et al. Zanthoxylum Alkylamides Ameliorate Protein Metabolism in Type 2 Diabetes Mellitus Rats by Regulating Multiple Signaling Pathways. 2021 Mar 11 WB ; Rat.
[IF=2.893] Lijiao Zhang. et al. Myostatin/HIF2α-Mediated Ferroptosis is Involved in Skeletal Muscle Dysfunction in Chronic Obstructive Pulmonary Disease. INT J CHRONIC OBSTR. 2022 Sep;17:2383-2399 WB ; Mouse.
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| 研究領(lǐng)域 | 細(xì)胞生物 干細(xì)胞 泛素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 2A3 |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 42 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human Fbx32: 1-200/355 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antib |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Fbx32 is an E3 ubiquitin ligase that initiates ATP dependent ubiquitin-mediated proteolysis and promotes muscle atrophy. It is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. It is also thought to recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation during skeletal muscle atrophy. Fbx32 interacts with MyoD by ubiquitination via a sequence found in transcriptional coactivators and therefore may play an important role in the course of muscle differentiation by determining the abundance of MyoD. Function: Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. Subunit: Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex SCF(FBXO32) formed of CUL1, SKP1, RBX1 and FBXO32. Subcellular Location: Cytoplasm. Nucleus. Note=Shuttles between Cytoplasm and the nucleus. Tissue Specificity: Specifically expressed in cardiac and skeletal muscle. Similarity: Contains 1 F-box domain. SWISS: Q969P5 Gene ID: 114907 Database links: Entrez Gene: 114907 Human Entrez Gene: 67731 Mouse Omim: 606604 Human SwissProt: Q969P5 Human SwissProt: Q9CPU7 Mouse Unigene: 403933 Human Unigene: 292042 Mouse Unigene: 72619 Rat |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Fbx32 monoclonal antibody, unconjugated (bsm-54451R) at 1:10000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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