| 產(chǎn)品編號 | bsm-34028M |
| 英文名稱 | ADGRE1 (F4/80) Mouse mAb |
| 中文名稱 | 小鼠抗表皮生長因子樣激素受體1（EMR1）單克隆抗體 |
| 別 名 | F4/80(mouse); Adhesion G protein-coupled receptor E1; Cell surface glycoprotein EMR1; Cell surface glycoprotein F4/80; DD7A5 7; Egf like module containing mucin like hormone receptor like 1; Egf like module containing mucin like hormone receptor like sequ |
 |
Specific References (8) | bsm-34028M has been referenced in 8 publications.
|
| 研究領(lǐng)域 | 細胞生物 免疫學 生長因子和激素 G蛋白偶聯(lián)受體 糖蛋白 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 3D4 |
| 交叉反應 | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應用 | WB=1:100-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 95 kDa |
| 檢測分子量 | 100-100 |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 200ug/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse F4/80 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M PBS (pH7.4) with 0.1% BSA, 0.02% Proclin300. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The epidermal growth factor (EGF)-TM7 family constitutes a group of class B G-protein coupled receptors, which includes CD97, EMR1 (EGF-like molecule containing mucin-like hormone receptor 1, designated F4/80 in mouse), EMR2, EMR3, FIRE, and ETL (1–3). These family members are characterized by an extended extracellular region with several N-terminal EGF domains, and are predominantly expressed on cells of the immune system (1–3). The EGF-TM7 protein family are encoded by a gene cluster on human chromosome 19p13 (1,3,4). The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria, and Langerhans cells in the skin (1). F4/80/EMR1 undergoes extensive N-linked glycosylation as well as some O-linked glycosylation (5,6). The function of F4/80/EMR1 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration, or as a G-protein coupled signaling component of macrophages. Function: Could be involved in cell-cell interactions. Subunit: Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. Contains 6 EGF-like domains. Contains 1 GPS domain. Subcellular Location: Cell membrane. Tissue Specificity: Wide expression; increased levels in peripheral blood mononuclear cells. Similarity: Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. Contains 6 EGF-like domains. Contains 1 GPS domain. SWISS: Q61549 Gene ID: 13733 Database links: Entrez Gene: 2015 Human Entrez Gene: 13733 Mouse Omim: 600493 Human SwissProt: Q14246 Human SwissProt: Q61549 Mouse Unigene: 2375 Human Unigene: 2254 Mouse |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human HL-60 cell lysates
Primary: Anti-ADGRE1 (F4/80) (bsm-34028M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 95 kDa
Observed band size: 120 kDa
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
