| 產品編號 | bs-6316R-BF647 |
| 英文名稱 | Rabbit Anti-Prostaglandin E Receptor EP1/BF647 Conjugated antibody |
| 中文名稱 | BF647標記的前列腺素EP1受體抗體 |
| 別 名 | Prostaglandin E Receptor EP1; EP1; PGE receptor EP1 subtype; PGE2 receptor EP1 subtype; Prostaglandin E receptor 1 subtype EP1 42kDa; Prostaglandin E receptor 1 subtype EP1; Prostaglandin E2 receptor EP1 subtype; Prostanoid EP1 receptor; PE2R1_HUMAN. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 細胞生物 信號轉導 轉錄調節(jié)因子 G蛋白偶聯(lián)受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse, Rat, (predicted: Human, Dog, ) |
| 產品應用 | Flow-Cyt=1ug/Test IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 42kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PTGER1/Prostaglandin E Receptor EP1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: PTGER1 is a subtype 1 receptor for prostaglandin E2 (PGE2). This receptor is coupled to the phosphatidylinositol-calcium second messenger system by G(q) proteins. PTGER1 may be an important modulator of renal function and is implicated in the smooth muscle contractile response to PGE2 in various tissues. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Abundant in kidney. Lower level expression in lung, skeletal muscle and spleen, lowest expression in testis and not detected in liver brain and heart. Similarity: Belongs to the G-protein coupled receptor 1 family. Database links: Entrez Gene: 5731 Human Entrez Gene: 19216 Mouse Omim: 176802 Human SwissProt: P34995 Human SwissProt: P35375 Mouse Unigene: 159360 Human Unigene: 474430 Mouse Unigene: 11423 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品圖片 |
Blank control (Black line):Mouse blood(Black).
Primary Antibody (green line): Rabbit Anti-Prostaglandin E Receptor EP1 antibody (bs-6316R-BF647)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-AF647 .
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
