| 產(chǎn)品編號 | bs-6940R-BF647 |
| 英文名稱 | Rabbit Anti-KDEL/BF647 Conjugated antibody |
| 中文名稱 | BF647標記的賴氨酸，天冬氨酸，谷氨酸，亮氨酸相關(guān)序列抗體 |
| 別 名 | KDEL; Lys Asp Glu Leu antibody; Lys-Asp-Glu-Leu. |
| 規(guī)格價格 | 50ug/1480元 購買 100ug/2680元 購買 大包裝/詢價 |
| 說 明 書 | 50ug 100ug |
| 研究領(lǐng)域 | 細胞生物 免疫學 細胞類型標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse, Rat, (predicted: Human, Dog, Pig, Cow, Rabbit, Guinea Pig, ) |
| 產(chǎn)品應用 | Flow-Cyt=1ug/Test IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 78kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide KDEL |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Endoplasmic reticulum Marker. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins (GRP 78) and GRP 94 respectively and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor. Subunit: Interacts with DNAJC1 (via J domain). Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with TMEM132A and TRIM21. May form a complex with ERLEC1, OS9, SEL1L and SYVN1. Subcellular Location: Endoplasmic reticulum. DISEASE: Note=Autoantigen in rheumatoid arthritis. Similarity: Belongs to the heat shock protein 70 family. Database links: Entrez Gene: 79070 Human Entrez Gene: 72050 Mouse Omim: 611613 Human SwissProt: Q6UW63 Human SwissProt: Q9JHP7 Mouse Unigene: 408629 Human Unigene: 281714 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 內(nèi)質(zhì)網(wǎng)標志物。 |
| 產(chǎn)品圖片 |
Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-KDEL antibody (bs-6940R-BF647)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-KDEL/AF647 Conjugated antibody (bs-6940R-BF647)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-AF647 .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
